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Registros recuperados: 136
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Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) ArchiMer
Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe.
A novel method using 6-dimcthylaminopurinc (6-0MAP) has recently been demonstrated to be a good triploid inductor for bivalve molluscs. The effects of 6-DMAP concentration, timing of treatment after fertilization and treatment duration on survival and triploid induction of Crassostrea gigas (Thunberg) embryos were examined. An influence of sperm concentration was also detected and discussed. Survival to D-stage was inversely related to 6-DMAP concentration and the percentage of triploids was shown to be 6-DMAP dose dependent. Two optimal moments for treatment application were determined corresponding to extrusions of the first and second polar bodies. Optimal treatment of 450 µmol/l 6-DMAP beginning 15 min after fertilization over a 10 min period yielded...
Tipo: Text Palavras-chave: Genetic; Triploid induction; Crassostrea gigas; 6 DMAP; Triploid; Oysters.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-3198.pdf
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Oyster population genetics : understanding natural populations and tracing introductions ArchiMer
Lapegue, Sylvie; Boudry, Pierre.
Oysters are among the most familiar of all marine invertebrate taxa. However our knowledge of oyster phylogeny and systematics is fragmentary. This is principally due to the plastic growth patterns of these animals, which result in a wide range of overlapping, ecophenotypic variants that greatly reduce the value of analysis based on shell morphology. Besides that, many intentional or accidental anthropogenic transfers have emphasised this situation. In several cases of misclassification or misidentification of oysters, DNA molecular data, have provided valuable new insights on the tracing of introductions or more generally on the phylogeographic relationships between oyster species. We will illustrate this issue with recent case studies from our lab on...
Tipo: Text Palavras-chave: Genetic; Ostreola stentina; Crassostrea honkongensis; Crassostrea gigas; Crassostrea angulata; Oysters; Population genetics.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3475.pdf
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Pacific cupped oyster - Crassostrea gigas ArchiMer
Lapegue, Sylvie; Boudry, Pierre; Goulletquer, Philippe.
Originating from the north eastern Asia, Crassostrea gigas is endemic to Japan, but has been introduced and translocated, mainly for aquaculture purpose, into several countries, almost worldwide (1). In North America, the species can be found from Southeast Alaska to Baja California, while in European waters the species is cultured from Norway to Portugal as well as in Mediterranean Sea (Fig.1) (2). Biological characteristics make it suitable for a wide range of environmental conditions, although it is usually found in coastal and estuarine areas within its natural range. Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (3).
Tipo: Text Palavras-chave: Genetics; Ecology; Crassostrea gigas; Biology; Oysters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3322.pdf
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Performance of triploid Pacific oysters Crassostrea gigas (Thunberg) reared in high carrying capacity ecosystem : survival, growth and proximate biochemical composition ArchiMer
Goulletquer, Philippe; Joly, Jean-pierre; Gerard, Andre; Le Gagneur, Eric; Moriceau, Jacques; Peignon, Jean-marie; Heurtebise, Serge; Phelipot, Pascal.
Triploid oysters Crassllstrea gigas were: produced in 1990 by treating fertilized eggs with cytochalasin B (CS). Triploids, treated diploids, and controls were deployed early 1991 in a high carrying capacity ecosystem on the Eastern Coast of Normandy (France). A monthly monitoring in 1992 showed that triploids yielded significantly higher growth rate and biochemical composition. However, growth was more heterogeneous. No evidence was found for a length or weight bimodal frequency distribution within triploid groups after a 26 months rearing cycle. Triploids showed retardation of gametogenesis. Carbohydrates content in triploids remained almost constant (40%) from June to September. Their survival rates were significantly lower than controls. Moreover...
Tipo: Text Palavras-chave: Biochemical compostion; Survival; Carrying capacity; Triploid; Growth; Oysters; Crassostrea gigas; Composition biochimique; Survie; Capacité trophque; Triploïdes; Croissance; Huîtres; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-3088.pdf
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Phenotypic and genetic consequences of size selection at the larval stage in the Pacific oyster (Crassostrea gigas) ArchiMer
Taris, Nicolas; Ernande, Bruno; Mccombie, Helen; Boudry, Pierre.
The life histories of oysters in the genus Crassostrea, like those of most marine bivalves, are typified by high fecundity and low survival in nature. Rearing conditions in hatcheries however ensure optimized density, diet, and temperature. Hatcheries are becoming increasingly important for the production of juveniles in aquaculture, and their culture practices often include culling of slow growing larvae to reduce and synchronize the time taken to reach settlement. Because previous studies have found substantial genetic variation for early life developmental traits in Crassostrea gigas, these culling practices are likely to cause highly different selective pressures in hatcheries from those in the natural environment. We studied the phenotypic and genetic...
Tipo: Text Palavras-chave: Oysters; Larvae; Hatchery; Genetic diversity; Culling; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1677.pdf
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Physiological and molecular basis of gametogenesis in triploid Pacific oysters, Crassostrea gigas ArchiMer
Normand, Julien; Huvet, Arnaud; Fabioux, Caroline; Raguenes, Morgane; Boudry, Pierre.
Genetic improvement of the Pacific oyster, Crassostrea gigas, can be achieved through triploidisation, which leads to partially sterility, allowing higher growth and survival together with improved meat quality during the reproductive period. Most triploids are not fully sterile and the genetic and environmental factors involved in their partial fertility remained to be investigated. We examined gametogenesis by image analysis of histological cross sections in diploid (2n) and triploid oysters obtained either by crossing tetraploids and diploid broodstock (« natural » triploids: 3nn) or from second polar body retention (« chemical » triploids: 3nc). 5-month old triploid spat reared in a standard intensive nursery system only showed a reduction of 50% of...
Tipo: Text Palavras-chave: Gametogenesis; Triploid; Crassostrea gigas; Genetic; Oysters.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3327.pdf
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Problèmes posés par l'analyse des relations trophiques huîtres-milieu ArchiMer
Deslous-paoli, Jean-marc; Heral, Maurice; Zanette, Yohann.
A relation between the evolution of biochemical components of molluscs and the available amount of food is difficult to establish. In addition to energy relations we tried to use a factorial correspondance analysis in the study on relation between the evolution of biochemical reserves of tissues of Crassostrea gigas Thunberg and the nutritional ability of the environment. The analysis showed a negative correlation between storing of carbohydrate and lipid reserves and amounts of mineral seston. In the same way, an accumulation of these reserves varies with the biomass of phytoplanctonic populations.
Tipo: Text Palavras-chave: Marennes Oléron; Potential feeding; Oysters; Trophic relation; Marennes Oléron; Nourriture potentielle; Huîtres; Relations trophiques.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/acte-2939.pdf
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Relations entre apports terrigènes et conchyliculture dans les Pertuis Charentais ArchiMer
Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Guesdon, Stephane; Bechemin, Christian.
This work presents interactions between quantitative and qualitative river freshwater inputs and the shellfish farming (oyster and mussel) in the Pertuis Charentais. The quantity of freshwater (i.e. salinity) seems to have a weak influence on the shellfish farming contrarily to its quality determined by particulate and dissolved matters contained in the water. In autumn and winter, large precipitations have a "globally positive" effect amending the coastal ecosystem. Associated dissolved nutriments and the organic matter largely determine the quality of the coming spring growth for bred shellfish, itself controlling in turn the annual yield efficiencies. However, in winter their effects are postponed because of strong mineral load, low luminosity and...
Tipo: Text Palavras-chave: Conchyliculture; Huîtres; Moules; Apports terrigènes; Zone côtière; Pertuis Charentais.; Shellfish aquaculture; Oysters; Mussels; Terrestrial inputs; Coastal zone; Pertuis Charentais..
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00248/35964/34490.pdf
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Relationship between valve activity, microalgae concentration in the water and toxin accumulation in the digestive gland of the Pacific oyster Crassostrea gigas exposed to Alexandrium minutum ArchiMer
Haberkorn, Hansy; Tran, Damien; Massabuau, Jean-charles; Ciret, Pierre; Savar, Veronique; Soudant, Philippe.
The complexity of the relationships between Alexandrium minutum (Am.) concentration in the water ([A.m.](w)), Paralytic Shellfish Poisoning contamination in the digestive gland ([PSP](dg)) and valve behavior was explored in oysters Crassostrea gigas. Two experiments were conducted, during which oysters' valve behaviour were analyzed. Oysters, first acclimated for 10-days with the non harmful microalgae Heterocapsa triquetra (H.t.), were exposed to four microalgae mixtures at constant total concentrations of 10 x 10(3) cells ml(-1) (experiment-1) and 5 x 10(3) cells ml(-1) (experiment-2): 100% Am.; 50% A.m.-50% H.t.; 25% A.m.-75% H.t.; 100% H.t. At the end of experiment-2, [PSP](dg) were measured. At 10 x 103 cells ml(-1), the microalgal ingestion decreased...
Tipo: Text Palavras-chave: Harmful algae; Oysters; Behavioral responses; Algae concentration; Toxin accumulation.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00041/15195/12617.pdf
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Relative importance of family, site, and field placement timing on survival, growth, and yield of hatchery-produced Pacific oyster spat (Crassostrea gigas) ArchiMer
Degremont, Lionel; Bedier, Edouard; Soletchnik, Patrick; Ropert, Michel; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre.
Summer mortality has been reported in the Pacific oyster, Crassostrea gigas, for many years in different parts of the world. The causes of this phenomenon are complex. The multidisciplinary program "MOREST", coordinated by IFREMER, was initiated to understand the causes of summer mortality of Crassostrea gigas juveniles in France and to reduce its impact on oyster production. Within this program, three successive groups of bi-parental families were bred in a hatchery in 2001 and placed in the field during summer in three sites (Ronce, Riviere d'Auray, and Baie des Veys). This paper reports the relative importance of family, site and field placement timing for three characters of major importance for oyster production: survival, growth, and yield. At the...
Tipo: Text Palavras-chave: Juvenile; Crassostrea gigas; Oysters; Yield; Growth; Survival.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1693.pdf
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Réponses et adaptations de la charge énergétique adénylique et l'activité enzymatique digestive à l'emersion chez Crassostrea gigas ArchiMer
Moal, Jeanne; Samain, Jean-francois.
The effect of tidal emergence on the adenylate energy charge value was studied in Crassostrea gigas oysters in Marennes-Oleron Bay. The shot-term AEC responses to aerial exposure differed according to the period of the year. However, on a long-term basis, osters from three bathymetric positions exhibited the same AEC value, but had a different growth rate. The amylase increase observed for the most emerged oysters may play a role in the maintenance of energy equilibrium.
Tipo: Text Palavras-chave: Charge énergétique; Amylase; Emersion; Huîtres; Crassostrea gigas; Cycle de marée; ATP; Adenylic energy charge; Amylase; Emersion; Oysters; Crassostrea gigas; Tidal cycle.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00022/13285/10331.pdf
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Report about mollusc diseaes ArchiMer
Berthe, Franck.
Of the 75 laboratories contacted through the survey, 54 answered, 14 of which are partially or totally devoted to mollusc diseases. In fact, only 4 laboratories are devoted full-time to this work. Of the 14 laboratories that declared to work on the diagnosis of mollusc diseases, 12 stated that they worked with mussels, 12 with oysters, 9 with clams and 8 with other mollusc species. This is coherent with available production information. According to production data in 2001 (FAO source), the main mollusc species in the region are the blue mussel, Mytilus edulis (297,485 tonnes), the Mediterranean mussel, Mytilus galloprovincialis (131,014 tonnes), the Pacific cupped oyster, Crassostrea gigas (127,323 tonnes, an important part of which is produced on the...
Tipo: Text Palavras-chave: Bonamia existiosus; Bonamis ostreae; Bonamiosis; Marteilia sydneyi; Marteilia maurini; Marteilia refringens; Marteiliosis; Perkinsus marinus; Perkinsus olseni; Perkinsus atlanticus; Perkinsosis; Vibriosis; Herpes like virus; Oysters; Pathology; Mollusc diseases.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-3300.pdf
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Résultats du réseau microbiologique du bassin d'Arcachon de 1989 à 1995 ArchiMer
Cantin, Christian.
Après avoir rapidement présenté le Bassin d'Arcachon et son environnement (situation géographique et réseau hydrologique), et rappelé quelques données sur l'ostréiculture, le tourisme et le réseau d'assainissement, le réseau de surveillance microbiologique (REMI) est décrit depuis sa mise en place en 1989 (réseau principal) jusqu'à son évolution en 1995 avec la mise en oeuvre d'un réseau étendu. Les résultats des analyses effectuées mensuellement sur les huîtres, au cours de ces sept années, sont présentés pour chaque station sous forme graphique, répartis en quatre secteurs  géographiques. lls ont été interprétés pour chaque réseau en fonction des quatre zones de salubrité définis par l'arrêté relatif aux classements des zones de production, et, ensuite...
Tipo: Text Palavras-chave: Bactériologie; Huîtres; Bassin d'Arcachon; Bacteriology; Oysters; Bay of Arcachon.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00427/53819/54808.pdf
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Scientific Opinion on Norovirus (NoV) in oysters: methods, limits and control options ArchiMer
NoV is highly infectious, and there is no threshold infectivity limit for NoV detected by PCR. The probability of becoming infected increases with the dose but depends also on the characteristics of the organism, the food matrix and the host factors. The relationship between the number of infectious virus particles and the number of virus genome copies detected by quantitative PCR is not a constant, and it is important to realise that the infectious risk associated with low level positive oysters as determined by real-time PCR may be overestimated. Quantitative data on viral load from areas compliant with current EU legislative requirements (E. coli standards) during January-March 2010 in 3 selected member states, show that a viral limit of 100, 200, 500,...
Tipo: Text Palavras-chave: Norovirus; Oysters; Diagnostic methods; Microbiological criteria; Treatment; Control.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00066/17735/15256.pdf
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Seuil de production de pseudo-fécès de l'huître creuse du Pacifique : Crassostrea gigas (Thunberg) dans des conditions de faibles charges particulaires ArchiMer
Soletchnik, Patrick; Goulletquer, Philippe; Geairon, Philippe; Razet, Daniel.
This study is part of a comprehensive research program focusing on the oyster shellfish management in the Marennes-Oleron Bay: Within this program, a task aimed to develop a biological model of the Pacific cupped oyster Crassostrea gigas. Our study addressed the issue of pseudo-faeces production threshold under various diet quality levels. Pseudo-faeces sampling was carried out using particulate matter concentration below Il mg 1-1 The various modalities prompted us to define two pseudo-faeces production models. The first model was developed using natural seawater. The organic matter ratio ranged from 25 to 45% while the pseudo-faeces production threshold reached 2.9 to 4.4 mg 1-1 of total particulate matter. The second model was established using seawater...
Tipo: Text Palavras-chave: Marennes Oleron; Pseudo faeces; Crassostrea gigas; Oysters; Biological model; Marennes Oléron; Pseudo fécès; Crassostrea gigas; Huîtres; Modèle biologique.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-3087.pdf
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Sex determination in the oyster Crassostrea gigas - A large longitudinal study of population sex ratios and individual sex changes ArchiMer
Broquard, Coralie; Martinez, Anne-sophie; Maurouard, Elise; Lamy, Jean-baptiste; Dégremont, Lionel.
Understanding sex determination in the Pacific oyster Crassostrea gigas, a sequential hermaphrodite, can provide prospective on the evolution of sex-determining systems for comparative reproduction from an evolutionary perspective. Surprisingly, this mechanism is still poorly understood. To date, sex ratio and sex change have never been studied at the individual level for a large size group and long-term monitoring. To this purpose, we performed an ambitious individual long-term follow-up (6 years) on a large population (cohort 1: 7488 oysters) produced from wild oysters, as well as for a second population produced from the cohort 1 (cohort 2: 4320 oysters). All oysters were individually sexed from 2014 to 2019. For the cohort 1, our results showed a...
Tipo: Text Palavras-chave: Sex-ratio; Sex change; Oysters; Hermaphroditism; Crassostrea gigas.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00587/69868/67845.pdf
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Shellfish viruses ArchiMer
Renault, Tristan.
Mortalities in a number of commercially important mollusc species have been associated with the detection of viruses belonging to several families. The first description of a virus was in adult eastern oyters, Crassostrea virginica, with the detection of virus particles resembling members of the family Herpesviridae. Subsequently, mass mortalities in French stocks of adult Portuguese oysters, C. angulata, were associated with irido-like virus infection. Other viruses observed in molluscs are described as members of the families Iridoviridae, Papovaviridae and Reoviridae. Little information is available on viral infections that affect molluscs due to primarily to the inadequacy of diagnostic methods that are employed when mass mortality events occur. Most...
Tipo: Text Palavras-chave: Virus; Scallops; Picornavirus; Oyter herpesvirus 1; Oysters; Molluscs; Irido Like virus; Diseases; Herpesvirus; Clams; Bivalves; Birnavirus; Abalones.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4932.pdf
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Situation of wild beds of Crassostrea gigas regarding OsHV-1 µvar: spatiotemporal description of contamination in Pacific oyster wild beds in Marennes Oleron Bay ArchiMer
Pepin, Jean-francois; Soletchnik, Patrick; Lupo, Coralie; Geairon, Philippe; Seugnet, Jean-luc; Robert, Stephane; Bernard, Ismal; Le Moine, Olivier.
This study focuses on the situation of wild oyster beds of Crassostrea gigas against OsHV-1 μvar and presents a spatio-temporal description of the OsHV-1 contamination in wild oyster beds in the bay of Marennes-Oléron from a range of sampling based on a hydrodynamic model and analyzes conducted in 2010. The objectives were to describe wild populations of pacific oysters in Marennes Oleron bay; to characterize these populations regarding OsHV-1 prevalence for spat and adults in connection with the isolation level from reared stocks and the sampling periods.
Tipo: Text Palavras-chave: Virus; OsHV-1; Contamination; Oysters; Crassostrea gigas; Spatiotemporal description; Marennes Oleron Bay.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00105/21673/19251.pdf
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Small-scale spatial variability of food partitioning between cultivated oysters and associated suspension-feeding species, as revealed by stable isotopes ArchiMer
Dubois, Stanislas; Orvain, Francis; Marin Leal, Julio César; Ropert, Michel; Lefebvre, Sébastien.
Oyster culture structures support a host of epibionts belonging to the same suspension-feeding guild, which are considered to be potential competitors for food with cultivated oysters. In an intertidal shellfish ecosystem on the northern French coast, an approach based on stable isotopes (C-13 and N-15) was used to investigate intra- and interspecific food resource partitioning among cultivated oysters and the main associated wild sessile epibionts such as polychaetes, barnacles, mussels and ascidians. The main objective of the present study was to determine inter- and intraspecific food partitioning, along with small-scale spatial variability, within the guild of suspension feeders. We demonstrated that interspecific competition was limited among...
Tipo: Text Palavras-chave: Trophic plasticity; Spatial variability; Stable isotopes; Sessile epibionts; Oysters; Shellfish.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2626.pdf
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Study of a haplosporidian (Ascetospora), parasitizing the Australian flat oyster Ostrea angasi ArchiMer
Bachere, Evelyne; Chagot, Dominique; Tige, Gilbert; Grizel, Henri.
Considering the epizootics which affect the French stock of flat oysters, Ostrea edulis (Grizel, 1985), trials to introduce an Australian oyster, Ostrea angasi, have been undertaken. The spat produced in a hatchery from breeders imported from Australia were reared in the south ofBrittany and in Normandy (Bougrier et al., 1986). Following mass mortalities (65% of individuals) a haplosporidian was observed in this species. Sporogenesis of the parasite was studied using Iight and electron microscopy.
Tipo: Text Palavras-chave: Haplosporidian; Parasitology; Pathology; Ostrea angasi; Oysters.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-3112.pdf
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